| # | Eastern Conference | MP | W | D | L | G | GD | Pts | Form |
|---|---|---|---|---|---|---|---|---|---|
| 1. | Charleston | 13 | 10 | 2 | 1 | 36:9 | 27 | 32 | |
| 2. | Detroit | 13 | 8 | 4 | 1 | 15:5 | 10 | 28 | |
| 3. | Indy Eleven | 14 | 8 | 3 | 3 | 23:13 | 10 | 27 | |
| 4. | Tampa Bay | 12 | 8 | 2 | 2 | 28:14 | 14 | 26 | |
| 5. | Pittsburgh | 13 | 7 | 2 | 4 | 15:10 | 5 | 23 | |
| 6. | Rhode Island | 13 | 6 | 5 | 2 | 21:11 | 10 | 23 | |
| 7. | Louisville City | 13 | 6 | 3 | 4 | 18:16 | 2 | 21 | |
| 8. | Hartford Athletic | 12 | 5 | 6 | 1 | 18:15 | 3 | 21 | |
| 9. | Miami FC | 13 | 5 | 4 | 4 | 24:23 | 1 | 19 | |
| 10. | Birmingham | 14 | 3 | 7 | 4 | 20:18 | 2 | 16 | |
| 11. | Brooklyn | 13 | 3 | 3 | 7 | 14:16 | -2 | 12 | |
| 12. | Loudoun | 13 | 1 | 8 | 4 | 19:23 | -4 | 11 | |
| 13. | Sporting Jax | 13 | 2 | 3 | 8 | 20:38 | -18 | 9 | |
| # | Western Conference | MP | W | D | L | G | GD | Pts | Form |
| 1. | Sacramento Republic | 13 | 8 | 3 | 2 | 23:11 | 12 | 27 | |
| 2. | Lexington | 13 | 8 | 1 | 4 | 25:13 | 12 | 25 | |
| 3. | Colorado Springs | 13 | 7 | 3 | 3 | 23:15 | 8 | 24 | |
| 4. | FC Tulsa | 13 | 7 | 3 | 3 | 13:7 | 6 | 24 | |
| 5. | New Mexico | 13 | 7 | 3 | 3 | 21:16 | 5 | 24 | |
| 6. | Phoenix Rising | 13 | 6 | 4 | 3 | 23:18 | 5 | 22 | |
| 7. | El Paso | 12 | 6 | 3 | 3 | 21:19 | 2 | 21 | |
| 8. | San Antonio | 13 | 5 | 5 | 3 | 14:12 | 2 | 20 | |
| 9. | Orange County SC | 13 | 5 | 3 | 5 | 14:18 | -4 | 18 | |
| 10. | Las Vegas Lights | 13 | 4 | 5 | 4 | 14:14 | 0 | 17 | |
| 11. | Oakland Roots | 14 | 4 | 5 | 5 | 17:18 | -1 | 17 | |
| 12. | Monterey Bay | 13 | 4 | 3 | 6 | 17:20 | -3 | 15 |